esiSCRIBE

esiSCRIBE is a very versatile T7 RNA-polymerase-based in vitro transcription kit, suitable to produce single- or double-stranded RNA.

Applications of the produced single-stranded (ss)RNA include guide RNA for CRISPR/Cas9 experiments and hybridization probes for FISH (fluorescence in situ hybridization), RNA SELEX (systematic evolution of ligands by exponential enrichment), Northern blots etc.

The most popular application of double-stranded (ds)RNA transcribed with esiSCRIBE is RNA interference (RNAi).

The reliability of the esiSCRIBE kit and quality of the resulting dsRNAs was validated in a large-scale RNAi screen, which applied dsRNAs obtained with the esiSCRIBE kit (Kittler R. et al., Genome-wide resources of endoribonuclease-prepared short interfering RNAs for specific loss-of-function studies, Nature Methods, 2007, 4, 337-344.)

esiSCRIBE is easy to handle and easy to use: No special equipment is required. A detailed protocol and all necessary reagents are included.

esiSCRIBE – In Vitro Transcription Kit for 199.00 EUR

Catalog-#: T7-esiSCRIBE-100