Our esiFLEX sub-genomic collections of esiRNAs provide maximum flexibility in terms of composition and array. You can assemble your own custom esiRNA library by choosing 24 or more esiRNAs from our selection of 16,881 human and 14,068 mouse esiRNAs, which target transcripts encoding proteins. In addition, you may choose from 1,761 human and 643 mouse esiRNAs targeting long non-coding transcripts.
The esiFLEX libraries are delivered in 96-well plates, each well containing either 1.0, 2.5 or 5.0 µg of esiRNA. The customer is free to specify positions of esiRNAs on the plates.
All esiRNAs were designed based on the sequence annotations from the ENSEMBL database.
Quality Control: esiRNAs are designed using the DEQOR design algorithm. The PCR products used for production are tested via gel electrophoresis and identity is confirmed by DNA sequencing. Quality of esiRNA is validated by gel electrophoresis and concentrations are determined by measuring OD260 or fluorescence intensity.
Purification: RNaseIII-digested products are purified via column purification using anion-exchange chromatography, precipitated with isopropanol and washed with ethanol.
Format: All esiRNA are delivered in solution (resuspended in nuclease-free TE buffer) normalized to 50 ng/µl.
Storage and Stability: esiRNAs stored at -20°C are stable for 2 years. No decrease in stability was observed after 12 freeze/thaw cycles.
Shipment: Shipped in solution on dry ice in sealed 96-well plates.
Technical Data Sheet: esiFLEX libraries will be delivered with a technical data sheet, which includes the plate number and positions of esiRNAs, the esiRNA ID numbers, organism, amount (µg), concentration, lot #, and target sequence. esiFLEX libraries will be delivered with a CD containing the product details as given above.