The product esiOPEN represents fully flexible custom esiRNAs. Here, you may provide a transcript sequence (minimum of 500 bp) from any species. Based on our proprietary bioinformatics analysis, we will design and synthesize an esiRNA of maximum specificity and silencing efficiency for you.
esiOPEN standard scales are 20 µg or 50 µg.
Quality Control: esiOPENs are designed using the DEQOR design algorithm. The PCR products used for production are tested via gel electrophoresis. Quality of esiOPENs is validated by gel electrophoresis and concentrations are determined by measuring OD260 or fluorescence intensity.
Purification: RNaseIII-digested products are purified via column purification using anion-exchange chromatography, precipitated with isopropanol and washed with ethanol.
Format: All esiOPENs are delivered in solution (resuspended in nuclease-free TE buffer) normalized to 200 ng/µl.
Storage and Stability: esiRNAs stored at -20°C are stable for 2 years. No decrease in stability was observed after 12 freeze/thaw cycles.
Shipment: Shipped in solution on dry ice in individual tubes.
Technical Data Sheet: esiOPEN will be delivered with a technical data sheet, which includes the esiOPEN ID number, organism, amount (µg), concentration, lot #, and target sequence.