Our esiRNA libraries for non-coding transcripts are state-of-the-art silencing triggers ideal for large-scale loss-of-function screens. Currently, we offer a human and a mouse library consisting of 1,761 and 643 esiRNAs, respectively, targeting long non-coding transcripts.
The libraries are delivered in 96-well plates, each well containing either 1.0, 2.5 or 5.0 µg of esiRNA.
Custom arrays, and other quantities or plate formats are available upon request.
Quality Control: esiRNAs are designed using the DEQOR design algorithm. The PCR products used for production are tested via gel electrophoresis, and identity is confirmed by DNA sequencing. The quality of each esiRNA is validated by gel electrophoresis, and concentrations are determined by measuring OD260 or fluorescence intensity.
Purification: RNaseIII-digested products are purified via column purification using anion-exchange chromatography, precipitated with isopropanol andwashed with ethanol.
Format: All esiRNA are delivered in solution (resuspended in nuclease-free TE buffer), normalized to 50 ng/µl.
Storage and Stability: esiRNAs stored at -20°C are stable for 2 years. No decrease in stability was observed after 12 freeze/thaw cycles.
Shipment: Shipped in solution on dry ice in 96-well plates.
Technical Data Sheet: esiRNA libraries will be delivered with a technical data sheet, which includes the plate number and positions of esiRNAs, the esiRNA ID numbers, organism, amount (µg), concentration, lot #, and target sequence. esiRNA non-coding libraries will be delivered with a CD-ROM containing the product details as given above.