For large-scale loss-of-function screens, we offer iB-RNA libraries targeting transcripts from 9,391 Tribolium castaneum genes. The supreme quality of the libraries has been independently confirmed by a recent publication in a high-impact journal:
Schmitt-Engel et al., 2015, The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology. Nature Commun, 6: 7822
The libraries are delivered in 96-well plates, each well containing 10, 20 or 30 µg of iB-RNA, normalized to 1 µg/µl in a buffer suitable for injection. Other quantities or plate formats, and custom arrays are available upon request.
Prices upon inquiry.
Quality Control: iB-RNAs are designed using the DEQOR design algorithm. The PCR products used for production are tested via gel electrophoresis, and identity is confirmed by DNA sequencing. The quality of each iB-RNA is validated by gel electrophoresis, and concentrations are determined by measuring fluorescence intensity.
Purification: All iB-RNAs are precipitated with isopropanol and washed with ethanol.
Format: iB-RNAs are dissolved in the customer's choice of either injection buffer (1.4 mM NaCl, 0.07 mM Na2HPO4, 0.03 mM KH2PO4, 4 mM KCl, 1% (v/v) phenol red, pH 7.2), TE buffer, or water and normalized to 1.0 µg/µl.
Storage and Stability: iB-RNAs stored at -20°C are stable for two years. No decrease in stability was observed after 12 freeze/thaw cycles.
Shipment: Shipped in solution on dry ice in sealed 96-well plates.
Technical Data Sheet: iB-RNA libraries will be delivered with an a technical data sheet, which includes the iB-RNA ID number, amount (µg), concentration, lot #, and target sequence. iB-RNA libraries will be delivered with a CD-ROM containing the product details as specified above.