A secondary iB-RNA (iB-SEC) is the tool of choice for the validation of a phenotype. The iB-SEC targets the same transcript as the primary iB-RNA, but matches a different section of the target, hence also called NOF for non-overlapping fragment.
For more information please read: Schmitt-Engel et al., 2015, The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology. Nature Commun, 6: 7822
for 32.50 EUR
for 52.50 EUR
Quality Control: iB-RNAs are designed using the DEQOR design algorithm. The PCR products used for production are tested via gel electrophoresis, and identity is confirmed by DNA sequencing. The quality of each iB-RNA is validated by gel electrophoresis, and concentrations are determined by measuring fluorescence intensity.
Purification: All iB-RNAs are precipitated with isopropanol and washed with ethanol.
Format: iB-RNAs are dissolved in the customer's choice of either injection buffer (1.4 mM NaCl, 0.07 mM Na2HPO4, 0.03 mM KH2PO4, 4 mM KCl, 1% (v/v) phenol red, pH 7.2), TE buffer, or water and normalized to 1.0 µg/µl.
Storage and Stability: iB-RNAs stored at -20°C are stable for 2 years. No decrease in stability was observed after 12 freeze/thaw cycles.
Shipment: Shipped in solution on dry ice in individual tubes.
Technical Data Sheet: iB-RNA will be delivered with a technical data sheet, which includes the iB-RNA ID number, amount (µg), concentration, lot #, and target sequence.