The red flour beetle (Tribolium castaneum) has emerged as an attractive model system for developmental genetics. For this organism (and others), long double-stranded (ds)RNAs have shown to be a superior silencing triggers over siRNAs.
Therefore, Eupheria developed efficient dsRNA silencers specifically for knock-down experiments in Tribolium castaneum.
Our individual iBeetle (iB)-RNAs are ideal for the functional characterization of single genes. All dsRNAs were optimized by the DEQOR algorithm for maximum specificity and silencing efficacy. Currently, we have iB-RNAs targeting transcripts from 9,391 different genes in stock.
For more information please read: Schmitt-Engel et al., 2015, The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology. Nature Commun, 6: 7822
for 25.00 EUR
for 40.00 EUR
Prices for larger scales or custom concentrations upon request.
Quality Control: iB-RNAs are designed using the DEQOR design algorithm. The PCR products used for production are tested via gel electrophoresis, and identity is confirmed by DNA sequencing. The quality of each iB-RNA is validated by gel electrophoresis and concentrations are determined by measuring fluorescence intensity.
Purification: iB-RNAs are precipitated with isopropanol and washed with ethanol.
Format: iB-RNAs are dissolved in the customer's choice of either injection buffer (1.4 mM NaCl, 0.07 mM Na2HPO4, 0.03 mM KH2PO4, 4 mM KCl, 1% (v/v) phenol red, pH 7.2), TE buffer, or water and normalized to 1.0 µg/µl.
Storage and Stability: iB-RNAs stored at -20°C are stable for 2 years. No decrease in stability was observed after 12 freeze/thaw cycles.
Shipment: Shipped in solution on dry ice in individual tubes.
Technical Data Sheet: iB-RNA will be delivered with a technical data sheet, which includes the iB-RNA ID number, amount (µg), concentration, lot #, and target sequence.