esiCLEAN is a purificaton kit for in vitro transcribed single guide (g)RNA. esiCLEAN frees your gRNA of nucleotides, buffer reagents and other contaminants originating from your in vitro transcription reaction. You obtain high yields of ultra-clean gRNA ready for your transfection experiments!
There is no need for a fume hood, as no volatile toxic solvents such as phenol or chloroform are involved.
25 preps for 187.00 EUR
Catalog-#: esiCLEAN-25
Suitable for purification of single-stranded RNA molecules of 100-120 nucleotides, such as single guide RNAs. The purification principle is anion-exchange chromatography.
All kit components are stored at room temperature.
Spin Columns | 25 |
Collection Tubes (2 mL) | 50 |
esiResin | 5.5 ml |
Equilibration Buffer | 30 ml |
Wash Buffer | 15 ml |
Elution Buffer | 18 ml |
RNase-free water | 1.5 ml |
3 M Sodium Acetate, pH 5.4 | 1.5 ml |